The Autoclave: How It Works the Different Varieties Available and the Precautions Taken

Your employer mandates that an aseptic environment be maintained at all times in the tissue culture lab that you work in. But can you walk me through the specifics of how these devices or machines work? Which variants of them are available, and how do the available options differ from one another in terms of their characteristics?




With these questions in mind, we are going to write a series of articles about the equipment that is utilized in tissue culture laboratories, as well as the manner in which they carry out their respective functions. When you have finished reading this article, you will have a complete understanding of autoclaves, including their various functions and the applications for which they are used.



In the year 1879, Charles Chamberland is credited with developing what is now known as the autoclave. The first modern autoclave wasn't created until the following year, in 1933.


The autoclave employs a method of sterilization that is predicated on the idea of moist heat as the driving force. Because of the high pressure inside the chamber, the temperature of the water is raised to a level at which it is able to more effectively sterilize the equipment.

Regardless of the size of the autoclave, the process of sterilization in an autoclave consists of three iterative phases, which are described in the following order:

During this stage of the process, any air that might be trapped inside the hermetically sealed chamber is evacuated with the help of steam that is supplied by the sterilizer.

At the beginning of the exposure phase, the exhaust valve is locked in its closed position.

Exhaust phase: the exhaust valve is opened, steam is emptied out of the chamber, and the temperature is brought back down to normal.


The outer chamber of a firearm is typically made of iron, while the inner chamber is typically made of stainless steel or gunmetal. The iron case is used to construct the outer chamber.

The autoclaves that are utilized in medical facilities and research establishments are designed to contain a jacketed chamber that is subsequently filled with steam. As a consequence of this, you are at liberty to acquire the device in accordance with the requirements that you set forth.

2. In addition to the pressure gauge, the whistle, and the safety valve, this product also includes these three additional components:

Not only does the pressure gauge show how much pressure has built up inside the autoclave, but it also makes sure that the machine and the working conditions are safe. The autoclave has a very thin layer of rubber that can rupture in the event that it is unable to carry out its functions. This will allow the pressure that is contained within the chamber to be released. It guards you against the risk of being injured in an explosion that was caused by an autoclave.

3. The room has a built-in electric heating system that raises the temperature of the water in order to generate steam. Always check to see that the generator has the correct amount of water in it to ensure that the process continues to go smoothly and to protect the autoclave parts from becoming overheated or burned.

4. If you skip this step, the equipment you're using won't be able to be sterilized.




On the market today, there is a wide selection of autoclave supplier available in a variety of configurations to fulfill your needs


  • This autoclave makes use of gravity and a drain port to introduce steam into the chamber, which ultimately results in the displacement of air within the chamber

An advanced autoclave, also known as the B-type, is of the positive pressure displacement type. This type of autoclave sterilizes equipment by first producing steam in a separate steam generator and then directing that steam into the autoclave itself. The positive pressure displacement type process refers to this method of operation. It comes outfitted with a vacuum generator as well as a steam generator, both of which operate effectively to ensure that the equipment is thoroughly sterilized. The combination of these two generators is what makes it possible for it to do so.


Do not make an attempt to sterilize liquids or solids that are impervious to water, such as oil or powder.

Only autoclavable bags should be used for the process of sterilizing waste from packages by using an autoclave.

Use autoclavable bags to sterilize your equipment.

Do not completely fill the chamber of the autoclave with the substance being sterilized.

Never place anything that could catch fire, is reactive, corrosive, toxic, or radioactive inside of an autoclave. This includes the bleach that is used in households and tissue that has had paraffin embedded in it.

The information that is presented in the following paragraphs, which relates to autoclaves, is brief and to the point. In addition to this, you are aware of the issue in the event that it manifests itself!

Posted in Default Category on December 26, 2022 at 03:26 AM

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